The length of the sequence between the two primers was expected to be approximately 1042bp.A fragment about 1042bp in length was amplified by RT-PCR using total RNA of Fasciola hepatica as templates.

• 从广西牛肝脏胆管中取出活的肝片吸虫，冷冻条件下快速磨成匀浆，抽提出总RNA，经反转录-聚合酶链式反应（RT-PCR）扩增出FheCL1的特异性片段。