The influence of mycelial age, enzyme system, enzymolysis time and temperature on the preparation and regeneration of Aspergillus niger UCo-3 protoplasts were studied by orthogonal design.

• 对菌龄、酶浓度、酶解温度、酶解时间四因素进行正交试验,确定原生质体形成与再生的最佳条件。