The human BTC can be successfully obtained from the pET32a(+) system with the biological activity of stimulating the proliferation of NIH3T3 cells.

• 人成熟BTC蛋白在pET32a(+)表达系统中得到高效表达,所纯化蛋白具有促进NIH3T3细胞体外增殖的作用。