The high performance liquid chromatographic methods with pre-column derivatization for the determination of amino acids were developed, while OPA and DNFB were used as the derivatization agents.

• 分别以邻苯二甲醛(OPA)及2，4-二硝基氟苯(DNFB)为衍生化试剂，发展氨基酸的柱前衍生HPLC分析方法。