The entire coding sequence was cloned into the pTO-T7 expression vector and expressed in E coli BL21. The expression yield of GFPxm was amounted to 50% of the total protein. Compared with GFP of A.

• 将gfpxm克隆至pTO-T7表达载体，GFPxm在大肠杆菌BL21中的表达量达菌体总蛋白的50%25左右。