The effects of DDN on the growth inhibition were exammed by MTT,and the apoptosis index (AI) were measured by TUNEL.

• 方法 1、以不同浓度DDN分四组[即空白对照组、DDN10uM组、DDN15uM组、DDN20uM组]处理hela细胞： 运用MTT法观察细胞增殖力，TUNEL法测定细胞凋亡指数AI（apoptosis index）以考量DDN抗肿瘤作用的效能；