The effect of potential scan direction and range, the presence of N_2 and O_2, electrolyte, the concentration of NaOH, KBr and fluorescein on fluorescein ECL was examined.

• 除ECL-3以外的所有发光峰都是由于荧光素的电氧化或荧光素与溶液中不同电位下电生成的物质之间发生反应产生(~1O_2~*)_2，(~1O_2~*)_2将能量传递给溶液中的荧光基团如荧光素或由荧光素电解生成的曙红Y，产生光发射。