The differentiation rate of callus produced by the pathway of de-differentiation was low, which was disadvantage to tissue culture of Zingiber offcinai Roscoe.

• 不定芽的直接分化途径可作为生姜组织培养的主要途径； 脱分化途径产生的生姜愈伤组织分化频率低，不利于生姜组织培养。