The crude preparation was obtained by ultrafiltration, three volumes of ethanol precipitation and acetone drying, and purified by resin 122#(H), Amberlite resin IR-45(OH) and Sephadex G-150 chromatography.

• 发酵液经超滤浓缩、乙醇沉淀、弱酸性阳离子交换树脂和弱碱性阴离子交换树脂去杂蛋白后,又经Sephadex G-150纯化得到PG。 PG经醋酸纤维膜电泳、聚丙烯酰胺凝胶电泳和Sepharose 4B柱层析,证明是单一均匀的糖蛋白。