The coupling ratio values of hapten to BSA and OVA by active ester reaction and diazotizition reaction were 12,2 and 30,1 respectively.

• 结论全抗原偶联成功,活性酯法和重氮化法使氯霉素半抗原与BSA,OVA的偶联比值分别为12,2; 30,1。