The clinical index including PLI, SBI, PPD, CAL were recorded, and the concentrations of RANKL and OPG in gingival crevicular fluid before and after periodontal treatment were determined using ELISA technique.

• 方法选择广泛型侵袭性牙周炎患者30例,分别在治疗前、治疗后2月和4月进行临床指标检测,并用滤纸条法收集治疗前后龈沟液样本,ELISA法检测RANKL和OPG浓度。