The chimerism and functional expression of mdr1 gene in bone marrow mononuclear cells were detected by polymerase chain reaction (PCR), Streptavidin-Peroxidase immunohistochemistry (IC) staining anddaunorubicin (DNR) efflux assay in the different aspects.

 
  • 采用PCR方法、免疫组化染色法、柔红霉素排出试验分别从基因、蛋白质和功能水平检测mdr1基因在兔骨髓单个核细胞中的功能性表达情况,探讨转染时间和转染效率、功能之间的关系。
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