The cells were resuspended, dispensed and cultivated in minimum essential medium containing 10% calf bovine serum. DEF monolayer usually was ready for use 36 hours after incubation.

• 10日龄鸭胚经胰酶消化和机械分散后,其成纤维细胞在含10%25小牛血清的MEM营养液中生长36h左右后成为致密的细胞单层。