The cells from the human fetal cerebral hemispheres at 10 - 22 weeks of gestation were placed into growth medium treated wiih epidermal growth factor (EOF, 20ng/ml) and basic fibroblast growth factor (bFGF, 20ng/ml) alone or in combination.

• 取胎龄10-22周的大脑半球,分散细胞后种于添加表皮生长因子(EGF,20ng/ml)和/或碱性成纤维生长因子(bFGF,20ng/ml)的培养基中。