The brain energy charge was calculated, and the activities of succinate dehydrogenase(SDH), cytochrome C oxidase(CCO) and Na+ - K+ - ATPase were measured with biochemical methods and high performance liquid chromatography(HPLC).

• 采用高效液相色谱法和生物化学法分别测定脑细胞能量负荷值和脑组织琥珀酸脱氢酶(succinatedehydrogenase,SDH),细胞色素氧化酶(cytochromecoxidase,CCO),Na+-K+-ATPase活性。