The artificial immunogen was prepared by linking DES and carrier protein BSA through isobutyl chloroformade method. The artificial-covering antigen was produced by the carbodiimide method to connect hapten DES and carrier-protein OVA.

• 己烯雌激酚人工抗原主要采用混合酸酐反应(氯甲酸异丁酯法)将半抗原己烯雌酚与载体蛋白牛血清蛋白连接制备人工免疫原,并采用碳二亚胺法将半抗原己烯雌酚与载体蛋白卵清蛋白连接制备人工包被原。