The approach is based on the principle that the a pa rticle will form double points on the image plane when the particle is out of fo cus in an optical system with a two pinhole lens.

• 该方法是当利用双孔透镜成像时，单点粒子在离焦光学成像系统中会形成包含粒子空间信息的两点像，根据粒子像在像平面上的位置和间距可以获取空间粒子的三维空间位置。