The activity analysis of NtCBKs: NtCBKs were expressed in BAC-To-BAC system, and purified by Ni-NTA and CaM-sepharose.

• 激酶生化特性分析：利用真核细胞表达系统BAC To BAC表达系统表达两烟草钙调素结合蛋白激酶，并经Ni-NTA和CaM-sepharose两步纯化后，用于激酶活性的分析。