The Xanthium sibiricum callus was induced on the MS culture medium attaching different kind and concentration hormone by Xanthium sibiricum blade, petiole and stem as explants.

• 不同组合及浓度配比的培养基对愈伤组织的诱导率存在显著差异,苍耳对2,4-D敏感,不宜用来诱导愈伤组织;