The PPV 7909 and PPV NADL-2 were grown on PK-15 cell lines, harvested and concentrated against polyethylene glycol 6000 overnight after freezing, thawing and ultrasonication as 80% Cytopathic effect (CPE) appeared.

• 本试验通过PK-15细胞增殖猪细小病毒(PPV)，待细胞出现80%25病变时，收获病毒细胞培养物，经反复冻融、超声波处理后，利用聚乙二醇6000浓缩病毒，葡聚糖G-200凝胶过滤的方法纯化病毒，得到了纯度较高的PPV。