The CCID50 was calculated by Reed - Muench formula. [Results] CCID50 increased with the culturing time and reached its peak on day 18, which was about 1 week earlier than that of conventional cell culture ELBA.

• [结果]随着培养时间的延长,感染性滴度逐渐增高,18d可达增长高峰,比常规组织培养ELISA法提前1周左右;