TK gene was 534bp and encoded 178 amino acids. On the N terminal of TK, ATP motif was found. TK gene was conserved in CPV, the identity of GTPV-TY TK gene compared with TK gene in other CPV strains were more than 96%.

• 试验中首先用PCR方法克隆了GTPV-TY的TK基因,TK基因ORF大小为534bp,编码178个氨基酸,TK的N端具有ATP结合结构域,与其它毒株的TK基因的核苷酸序列比较显示CPV各毒株TK基因的同源性大于96%25,保守性很高。