Succinimide and its enzymatic product succinamic acid in the presence of a cyclic imidase were analyzed by high performance liquid chromatography(HPLC).

• 该方法既可用于定量分析琥珀酰亚胺和琥珀酰胺酸,又能用于测定环二酰胺酶的活性及产物转化率。