Substrate was catalysed by immobilized lipaes to produce fatty acid and fatty acid was captured by CaCl 2 to form precipitate MCOOCa.Precipitation deposited active site of immobilized lipaes.

• 结果表明:以合成出的大孔吸附树脂为固定化酶载体,酶活较高,活性脂肪酶分布较均匀。