Submandibular tissues of rats were incubated in vitro and different concentration of FSH was added.Enzyme link immunoassay was used to measure EGF and NGF in supernatant.

• 体外孵育大鼠下颌下腺组织并给予不同浓度FSH,应用酶联免疫分析方法检测上清液中NGF和EGF的含量。