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- Abstract:Objective To investigate the effects of exogenous MTH gene stably transfection on growth of lung adenocarcinoma cell A549 in vitro. 目的研究外源性人金属硫蛋白H(MTH)基因稳定转染对肺腺癌细胞A549生长的影响及其机制。
- The chemotaxis and calcium mobil i zation assay were used for examining bioactivities of the cells stably transfect ed with CCR4. 通过基因转染技术获得稳定转染细胞株,利用趋化实验、钙流实验证实稳定转染细胞株能有效表达CCR4并具有生物功能。
- RT-PCR showed that E1A gene had a stable transfection in Hela cells and obviously down-regulated Her-2 expression. RT-PCR结果显示:E1A基因在Hela细胞中能稳定表达,E1A基因的转染明显降低了Her-2基因在Hela细胞中的表达水平;
- Methods: PC12 cells were stably transfected with pEGFP-C2/5-LOX (GFP/5-LOX) or pEGFP-C2 vectors (control). 方法:将带有5-LOX基因的pEGFP-C2质粒及pEGFP-C2质粒(对照),稳定转染至PC12细胞;
- RT-PCR demonstrated that E1A gene had a stable transfection in Hela cells and obviously down-regulated HER-2/neu expression. RT-PCR结果显示:E1A基因在宫颈癌细胞中能稳定表达并且显著降低HER-2/neu在宫颈癌细胞中的表达水平。
- Stably transfected and control Hela cell lines were injected subcutaneously into female Balb/c/nu mice. 稳定转染Ang-2的细胞注射Balb/c裸鼠皮下。
- MMP-9 activity was assessed in supernatants from HBECs stably transfected with pEGFPc1 -antisense-ET-1 converting enzyme(ECE) RNA. 明胶聚丙烯酰胺凝胶电泳酶谱法检测稳定转染ET - 1转换酶 (ECE)反义核酸上皮细胞培养上清MMP - 9酶活性。
- Stable transfection of FP248 gene to the SMMC-7721 cell line demonstrated a promoting effect on the proliferation of SMMC-7721 cell line in vitro and in vivo. 由此可见,FP248对SMMC-7721细胞的生长具有促进作用,以上的实验给了我们一致性的结果,由此我们可以推断FP248在肝癌的发生发展中具有重要的作用。
- Compared with pcDNA3 vector,there was significant increase of peroxiredoxin 1 expression in the PC3 cells stably transfected with pcDNA3/Prx 1 monitored by Western blot analysis. Western blot分析表明,Prx 1真核表达质粒稳定转染的PC3细胞表达Prx 1显著增加;
- The positive cell clones had been obtained after selected by G418. The stable transfection and expression of AIRE in HeLa cells were determined by the fluorescence microscope, FACS and Western blotting. 继续培养 4周 ,用荧光显微镜观察、流式细胞仪 ( FACS)检测和免疫印迹法检测 AIRE基因在 He La细胞中的稳定表达情况。
- CMV.Delta4.FLAG was constructed and transfected into COS7cells transiently,and then stably transfected into CHO cells. The high-expressing Delta4-CHO clones were set up and screened. CMV.;Delta4
- METHODS: Bcl-XL siRNA and negative siRNA plasmid vector were stably transfected into A549/DDP cells. Drug sensitivity of the cells to cisplatin(DDP) was analyzed with MTT and flow cytometry. 方法:将Bcl-XLsiRNA质粒载体转染至A549/DDP细胞,MTT、流式细胞仪检测细胞药物敏感性的改变;
- Keywords hydatidiform mole;F10 gene;stable transfection; 葡萄胎;F10基因;稳定转染;
- The only successful study of ribozymes against HBV in cells was shown in a stable transfectant that endogenously produced hairpin ribozymes. 唯一在细胞内核酶成功抑制HBV的研究是转入内源性稳定表达系统来表达发夹样核酶。
- Stably transfection 稳定转染
- Real unity stays stably in advance. 真正的统一在进步中持续。
- Vegetable can grow on the stably established table. 蔬菜能在稳定放置的桌子上生长.
- This way, we can stand more stably. 韩佳:这样可以站得稳一点。
- The system runs stably in a uptown in Beijing. 本系统已在北京某小区稳定运行.
- Keywords IBP;siRNA;Stable transfection;Apoptosis;Immunological homeostasis; 稳定转染;细胞凋亡;免疫自稳;
- 今日热词
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries