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- Southern blotting method 萨瑟恩[蛋白质]印迹法
- Southern blot technique developed to identify DNA fragments. 印迹杂交被用来发现DNA片段。
- We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization. 我们用常见的克隆载体pBR322作探针,用核酸点杂交和Southern吸印方法,检测了来自173人的213例样品。
- Method:Using Western blot method to measure HSP of hypothalamus in febrile and hypothermal rats. 方法：取高、低体温大鼠下丘脑组织，应用蛋白印迹法测定热休克蛋白含量。
- Southern Blot with positive plants of PCR showed that CMO and BADH have been integrated into the rice genome. 对随机抽取的PCR检测双阳性植株进行Southern blotting杂交检测分析，表明CMO和BADH基因均已整合到转基因水稻的基因组中。
- M ethod:Using Western blot method to measure HSP of hypothalamus in febr ile and hypothermal rats. 方法:取高、低体温大鼠下丘脑组织,应用蛋白印迹法测定热休克蛋白含量。
- Southern blot analysis showed that Oncidium has at least 4 copies of EIN2 in its genome. 南方墨点分析显示EIN2至少有四个拷贝分布于基因体组内。
- The presence or absence of particular restriction sits can be ascertained using DNA probes in the technique called SOUTHERN BLOTTING. 不同个体限制酶的位点有很大的不同，因此可以被用来分析和比较基因组。
- Southern blotting analysis of genomic DNA indicated that enolase was likely to be a low-copy gene in the oilseed rape genome. Southern杂交结果显示烯醇酶以低拷贝形式在油菜基因组中存在.
- The expression state of p53, Bcl-2 and Bax of rat hepatocytes BRL-3A as exposed to microcystin LR was studied with western blot method. 用蛋白印迹法研究了微囊藻毒素LR(MCLR)暴露时大鼠BRL-3A细胞p53、Bcl-2和Bax的表达情况.
- In this study the authors detected both nm23 H 1 and nm23 H 2 mRNA levels in 52 tissues samples of patients with carcinoma of buccal mucosa (CBM) by Northern blotting method. 通过Northern斑点杂交技术研究52例颊癌及相关组织中肿瘤转移抑制基因nm23－H1和nm23－H2mRNA的表达。
- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑点杂交法和 Southen 印迹法对脑瘤中 erbB、sis、myc 和 fos 这四种癌基因的扩增和重排进行了研究。
- Results of Southern blotting suggested that there were all a small gene family of MaMAPK, and MaMAPKgene may be small copies in genomic DNA of three species of Malus. Southern杂交结果表明，MoMAPK基因在3种苹果的基因组中均以低拷贝和基因家族形式存在，为进一步研究MaMAP基因的表达调控，揭示苹果属植物耐旱的分子机制奠定了基础。
- The cDNA probe hybridized with BVDV RNA, HCV RNA and yeast tRNA by the dot blot method The BVDV RNA and HCV RNA were positive and yeast tRNA was negative. 琼脂糖凝胶电泳结果说明它与BVDV RNA相似。 该cDNA的探针用斑点杂交方法与BVDV RNA,HCV RNA和酵母tRNA杂交,BVDV RNA和HCVRNA显阳性反应,酵母tRNA显阴性反应,结果说明合成的cDNA为BVDV RNA的cDNA。
- Finally, on the basis of the established transformation protocol, rolB-pttGA20ox genes were stably transferred to the Populus alba by PCR and Southern blotting tests. 最后在此基础上,进行rolB-pttGA20ox双价基因的转化,并由PCR、Southern杂交和形态特征差异检测,以证明是否为转化植株。
- In the present study, the effects ofglutamic acid and acetylcholine (ACh) on the induction of hsp70 mRNA in PC12 cellswere studied by Northem blot method. 本文以诱导型热休克蛋白（hsp）70的cDNA为探针，运用Northernblot的方法，在嗜铬细胞瘤细胞（PC12）上，分析了谷氨酸和乙酰胆碱对hsp70mRNA的诱导作用。
- PCR assay and Southern blot analysis showed that CMO gene was integrated successfully into the genome of transformed plant. PCR及Southern检测证明CMO基因已整合进抗性植株基因组;
- With photosensitivebiotin lablled probes,HEV derived from clone ET1.1(418 bp)and ETV derived from Echo-13(500bp),Dot and Southern blotting were carried out on the NC membranes. 以光敏生物素标记HEVET1.;14186p探针，对硝酸纤维素膜进行点杂交和Southern印迹杂交。
- Transgenic plants were confirmed by PCR, PCR-Southern, and genomic Southern blotting which showed that the bar and pin II genes were co-integrated into the plant genome. 获得的转基因植株经PCR、PCR-Southern杂交以及植物基因组Southern杂交证实,目的基因已经整合入植物基因组中。
- Transgenic potato plants 1M, 2M, 3M, 5M, 10M, llM provided by laboratory were analyzed by Southern blot and Northern blot. ELISA检测转基因马铃薯病毒抗性数据显示，温室条件下，3个转基因马铃薯株系(2M、3M、11M)具有病毒抗性，3个株系(1M、5M、10M)感病；
