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- Southern blot technique developed to identify DNA fragments. 印迹杂交被用来发现DNA片段。
- blot southern blot DNA 印迹
- Southern blot Southern 印迹, DNA印迹
- We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization. 我们用常见的克隆载体pBR322作探针,用核酸点杂交和Southern吸印方法,检测了来自173人的213例样品。
- Southern Blot with positive plants of PCR showed that CMO and BADH have been integrated into the rice genome. 对随机抽取的PCR检测双阳性植株进行Southern blotting杂交检测分析，表明CMO和BADH基因均已整合到转基因水稻的基因组中。
- Southern blot analysis showed that Oncidium has at least 4 copies of EIN2 in its genome. 南方墨点分析显示EIN2至少有四个拷贝分布于基因体组内。
- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑点杂交法和 Southen 印迹法对脑瘤中 erbB、sis、myc 和 fos 这四种癌基因的扩增和重排进行了研究。
- PCR assay and Southern blot analysis showed that CMO gene was integrated successfully into the genome of transformed plant. PCR及Southern检测证明CMO基因已整合进抗性植株基因组;
- Transgenic potato plants 1M, 2M, 3M, 5M, 10M, llM provided by laboratory were analyzed by Southern blot and Northern blot. ELISA检测转基因马铃薯病毒抗性数据显示，温室条件下，3个转基因马铃薯株系(2M、3M、11M)具有病毒抗性，3个株系(1M、5M、10M)感病；
- Southern blot analysis and PCR of target segment in the genome of tobacco showed that all of the plants generated were the transgenic plants. 转基因植株总DNA PCR和Southern blot分析表明目的基因已经整合到植物基因组中,但转基因的拷贝数与抗病性无明显的相关性。
- Dot blot and southern blot analyses suggested that gfp gene is integrated into the genome of transgenic plants of Nicotiana tabacum, Pentunia genome. 通过对有绿色荧光的烟草、矮牵牛进行dot blot、southern blot分析，都获得阳性杂交结果，证实gfp基因已整合到植物基因组中并与绿色荧光观察结果一致。
- Methods Accumulation of ciprofloxacin in clinical isolates of Escherichia coli was measured by fluorometry, and acrAB gene was identified by PCR and Southern blot. 方法 应用荧光法测定临床分离大肠杆菌对环丙沙星的主动外排功能 ; PCR及Southernblot测定主动外排系统AcrAB结构基因acrAB ;
- Methods Ordinary PCR, Southern blot and direct ISPCR were applied and compared in detecting Hib in 100 paraffin-embedded lung tissues of autopsy children died of CAP. 方法用普通PCR、Southern杂交和直接ISPCR三种方法检测100例死于肺炎的儿童尸检石蜡包埋肺组织标本中的Hib,分析其致病、致死及在肺组织中的分布情况,并对这些方法对比分析。
- Methods: The gene rearrangement in blood and bone marrow of 12 children with AML was detected by Southern blot hybridization using LE11. 8, MYO and M13 probes. 方法：用LE11.;8、MYO和M13探针，经Southern杂交法检测12例儿童急性粒细胞白血病患者的外周血或骨髓细胞的基因重排。
- Total DNA of the 2 pathogens and their similar ones digested with EcoR I, were hybridized with mtDNA probes of 2ul8 and 4u40 by Southern blot, the RFLP patterns could identify F. 利用Southern杂交技术;将致病菌和近似菌的基因组DNA分别用EcoRI 酶切;与mtDNA探针2u18和4u40杂交的RFLP图谱可成功的鉴定F.
- Conclusion By using RT-PCR, Southern blot and covalent cross-linking techniques, it was demonstrated that SGC 7901 cells can express bombesin receptor mRNA and protein. 本研究为探索蛙皮素和蛙皮素受体的拮抗剂应用于肿瘤研究以及蛙皮素作用后细胞内信息传递途径提供了基础。
- Southern blotting Southern 印迹(法), DNA印迹(法)
- DNA dot blot analysis showed that 73. 4% of resistant plants contained GNA and hpt genes. Southern blot analysis confirmed the integration of GNA gene in the genome of transgenic rice. DNA点杂交检测表明73.;4%25的抗性植株同时含有GNA基因和hpt基因;Southern杂交分析进一步证实了GNA基因在转基因水稻基因组中的整合
- Organogenic transgenic calli and transgenic regenerated plantlets were produced on selection medium containing 15mg/L kanamycin, and confirmed by GUS histochemical staining, polymerase chain reaction (PCR), and southern blot analysis. 器官发生的转基因愈伤组织和转基因的再生植株已经获得 ,并经GUS组织化学染色、聚合酶链式反应和Southern杂交分析证实。
- Methods The rearrangements of AML1 and ETO genes were detected by Southern Blot and the AML1-ETO fusion gene by nested RT-PCR combined with sequencing in K562 cells treated with etopside (Vp16) and doxorubicin(DOX). 方法 K5 6 2细胞经不同浓度足叶乙甙 (Vp16 )和阿霉素 (DOX)作用不同时间后用Southern杂交检测AML1、ETO基因重排 ,用筑巢式RT PCR联合序列分析检测AML1 ETO融合基因。
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries
