Some new type of two-hybrid systems,such as split-ubiquitin system, protein-fragment complementation assay, repressor reconstitution assay and SOS recruitment system, have been developed recently.

• 近年来,一些不依赖于转录因子活性的新型双杂交系统相继建立,如分离的泛素系统、蛋白质片段互补分析、阻遏物重构分析和SOS恢复系统等。