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- The anchorage independent growth (soft agar colony formation) appeared in the 15th passage of BEAS-2B? NNK cells. 第15代BEAS2BNNK细胞具有锚着独立性生长特性(软琼脂克隆形成);
- The positive clones were screened by G418.The proliferation,migration,and invasion of MDA-MB-231 cells before and after transfection were assessed by CFSE-flow cytometry,monolayer wound healing assay,and soft agar colony formation test. 应用CFSE-流式细胞仪检测,单层伤口愈合实验,软琼脂克隆形成实验等方法观察了MDA-MB-231细胞转染前后,细胞体外增殖及侵袭能力等指标的变化。
- soft agar colony formation assay 软琼脂集落形成
- Soft agar colony formation 软琼脂培养
- Leukemic colony formation unit (L-CFU) was cultured in soft agar medium in 10 cases with acute myeloblastic leukemia (AML) with PHA-LCM condition medium as a stimulating factor. 本文应用体外软琼脂培养技术,以PHA-LCM条件培养液作为白血病细胞集落形成刺激因素,对10例急性粒细胞白血病患者进行了L-CFU培养。
- Effect of profilin on cells colony formation in soft agar 原丝蛋白对细胞在软琼脂中集落形成的影响
- Keywords TIF3 gene;cadmium;proto oncogen;transformation;nude mouse inoculation;NIH3T3 cell;colony formation in soft agar; TIF3基因;镉;原癌基因;细胞转化;裸鼠;NIH3T3细胞;软琼脂集落形成;
- The colony forming efficincy in soft agar increased with the passaging. 软琼脂克隆形成率随着照后传代次数的增加而明显增高;
- The infected cells ( 293-BK cells ) grew as well as other 293 cells, and formed colony in soft agar 14 days later. 感染细胞(称作293-BK细胞)如其他293细胞一样生长;14天后可在软琼脂中形成细胞集落.
- Current inspection of transformed cells by the method of double-major in soft agar cloning experiments and tumorigenicity in nude mice experiments. 目前检验转化细胞致瘤性的方法主要有双层软琼脂克隆实验和裸鼠致瘤实验。
- Usually, tiny colonies of ureaplasma urealyticum on the agar plate are counted under microscope for obtaining colony formation unit/ml ( CFU/ml ) and the pH change of the media is determined with color change unit/ml ( CCU/ml ). 脲支原体的定量计数,因其个体微小,一般需要在光学显微镜下计数平板上生长的菌落,得出菌落形成单位(CFU/ml)和在试管中观察液体培养基pH改变,依颜色变化程度确定颜色变化单位(CCU/ml)。
- In this study, immortalized mesenchymal stem cell lines can be formed in soft agar cloning, cloning rate was only 2.1%, but not tumorigenicity in nude mice. 本实验的永生化间充质干细胞系可在软琼脂内形成克隆,克隆形成率只有2.;1%25,但不在裸鼠体内致瘤。
- Methods The effects of prazosin on the proliferation of K562 cells were evaluated by liquid culture assay, MTT assay and colony formation assay. 方法:采用四唑盐(MTT)比色实验、集落形成实验为指标观察哌唑嗪对K562细胞增殖作用的影响;
- Compared with control cells, the result of soft agar culture showed that the cloning efficiency of SACC-83 cell, which is transfected with NCAM gene, decreased significantly. 4.;软琼脂培养结果显示,与对照组相比,转染了NCAM基因的SACC-83细胞克隆形成率明显降低。
- The numbers of colony formation of progenitor cells in Evi1 and MDS1?Evi1?positive MDS patients were decreased as compared with Evi1 and MDS1?Evi1?negative patients. Evi1和MDS1?Evi1表达阳性的MDS患者祖细胞集落形成数低于Evi1和MDS1?Evi1阴性者。
- In the indices, CFU is Colony Formating Unit, NTU is the unit for turbidity. 水质指标单位中,CFU为菌落形成单位数NTU为浊度表示单位。
- In allthe three patients with RAEB-T with avery low colony formation and a high clus-ter: colony ratio transformed to acute mye-logenous leukemia and died. 3例RAEB-T型患者均转化为急性白血病死亡。
- The effect of Leukemia-associated inhibitor (LAI) in leukemia cells conditioned media (LCM) and sera from patients with ANLL on normal T lymphocyte colony formation were studied. 通过收集16例急性非淋巴细胞白血病(ANLL)病人的血清和外周血白血病细胞条件培养液(LCM),对血清和LCM中的白血病相关抑制物(LAI)对正常人T淋巴细胞集落(CFU-TL)形成的影响进行研究。
- The rates of ICM at-tachment, primary ICM colony formation and the efficiency of hESC derivation were similar between the ICMs isolated by the two methods (P>0.05). 两种分离内细胞团方法在内细胞团贴壁率、原始克隆形成率及建系率上没有显著性差异(P>0.;05),所有囊胚进一步培养、分离后建立了四株人类胚胎干细胞系。
- However, the time needed for colony formation in K-SFM medium was significantly shorter and the efficiency of colony formation was significantly higer than that in DMEM medium. 在K-SFM中2周时克隆形成率显著高于在DMEM中的克隆形成率。