Since the mutant lossing the first 6 amino acids from the N terminus of firefly luciferase keeps 77% of natural luciferase activity, the 7th N terminal amino acid is of great importance to the normal enzymatic function.

• 由于N末端缺失6个氨基酸的突变体保持了77%25的酶活性，因而萤火虫荧光素酶N末端第7个氨基酸与酶的催化活性密切相关。