Shoots regeneration frequency increased significantly when cotyledons were preconditioned in MSB5 medium with 1 mg/L 2,4-D and 1 mg/L BA for 3 days and then cultured in MMO medium containing 4.5 mg/L BA.

• 将子叶在附加1 mg/L BA和1 mg/L 2，4-D的MSB5培养基中预培养3天，再转入含有4.；5 mg/L BA的MMO培养基中培养，可显著增加芽再生率，并且可将再生周期比对照缩短1/3。