Sequence and restriction analysis revealed APN gene was cloned in frame into pGEX-4T,SDS-PAGE profile showed a clear protein band with a relative molecular weight of 135 000. Conclusion:Human APN gene was successfully cloned ?

• 重组质粒测序和酶切结果显示 :APN基因已正确插入 pGEX 4T ,重组蛋白及纯化产物SDS PAGE在 135 0 0 0处有一条明显的蛋白表达条带。