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- Keywords Streptococcus pyogenes;Gene typing;DNA polymorphism;Random primer PCR; 关键词酿脓链球菌;基因分型;DNA多态性;随机引物PCR;
- Random primer PCR technique 随机引物PCR
- Citrus genomic DNA library was bloted with the AP1 and FBP1 mixed MADS box genes probe labeled by random primer method. 以大三岛脐橙基因组DNA为材料 ,用拟南芥AP1和矮牵牛FBP1的混合质粒DNA为模板 ,采用随机引物法标记探针 ,对柑桔基因组DNA文库进行噬菌斑原位杂交。
- The particular band pattern of dark and light was observed by using random primer PRINS. 结果表明，随机引物延伸在染色体上呈现明暗相间的带纹样特征。
- Objective: (1) To establish PCR reaction system that uses allele-specific primer PCR technique to detect SNP of KLOTHO gene. 目的:(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
- Citrus genomic DNA library was bloted with the AP1 and FBP1 mixed MADS-box genes probe labeled by random primer method. 以大三岛脐橙基因组DNA为材料，用拟南芥AP1和矮牵牛FBP1的混合质粒DNA为模板，采用随机引物法标记探针，对柑桔基因组DNA文库进行噬菌斑原位杂交。
- The universal primer(CHS1 1S,CHS1 1R)is regarded as a specificity primer. The result showed that the sensitivity of universal primer PCR is 100 fg. 皮肤病原真菌通用引物(CHS1 1S,CHS1 1R)具有较强的特异性,敏感度为100 fg;
- Here we present the results of random primer and SOX degenerate primer PRINS on human metaphase chromosomes. 分别应用随机引物和SOX基因兼并引物在人类染色体上进行了原位延伸标记。
- Sensitivity determination of universal primer PCR: Seven samples ranging from 100 ng to 100 fg shown positive,except the 10 fg sample yielding negative band. 皮肤病原真菌通用引物PCR敏感性测定,模板DNA 100 ng~100 fg的7个系列浓度均扩增出了阳性条带,10 fg为阴性。
- The fingerprints amplification by polymerase chain reaction(PCR) with 6 polymorphic 10-based random primers selected from 40 ones was analyzed. 从40个引物(10碱基的寡核苷酸)中筛选出6个引物,对它们的PCR扩增结果进行分析。
- The comparison between PCR and random primer labeled method PCR和随机引物标记探针的方法比较
- Objective To investigate the possibility of applying degenerate oligonucleotide primer PCR(DOP-PCR) and comparative genomic hybridization(CGH) in analysing genomic genetics of a single cell. 目的探索单细胞退变寡核甘酸引物PCR(DOP-PCR)-比较基因组杂交(CGH)技术应用于单细胞全基因组分析及着床前胚胎遗传学研究的可能性。
- Methods: Optimal reaction system and single random primer of RAPD were used for genotyping of the 108 strains of EA isolated from the clinical samples and the genotype profile was drawn. 方法:以优化的反应体系和单一随机引物对临床分离的108株EA进行RAPD法基因分型并绘制基因分型图谱。
- random primer DNA labeling system 随机引物DNA标记系统
- Analyzed the genome DNA of high-protein wheat of T4 and CK by 34 random primers using RAPD. 选用34个随机引物对T4代高蛋白小麦和对照的基因组DNA进行分析。
- Soiled dishes were piled at random. 脏碟子胡乱地堆放着。
- Universal Primer PCR for Detection of Clinic Bacteria 通用引物PCR检测临床常见致病菌的实验研究
- The RNA was rerverse transcribed according to the illustration of cDNA first chain construction agent kit with random Primers Oligo dT. 由于其发病机理尚不完全清楚，早期诊断仍有困难。
- Total 67 random primers were selected in RAPD analysis.Sex differentiations of population level were found in 18 primers. 共筛选出67个RAPD随机引物，其中18个引物扩增出了群体水平的性别差异。
- HBV genotypes were determined by type-specific primers PCR method. PreC/BCP mutations were detected by direct sequencing of PCR products,and Clustal W 1.8 software was applied to analyzing the sequences. 以型特异性引物PCR法检测16对乙肝疫苗阻断失败的母婴和88例阻断成功的母亲血清样本中HBV基因型;用PCR产物直接测序法检测HBV前C/ BCP突变;采用Clustal W 1.;8软件进行序列分析。
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries
