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- The mRNA levels of this gene were determined in SCLC cell line SH77 ,big cell lung cancer cell line H460,lung adenocarcinoma cell line SPC A 1 and SPC A 1/CDDP using semi quantitative RT PCR assay. 应用半定量RT-PCR方法检验小细胞肺癌SH77、大细胞肺癌H460、肺腺癌SPC-A-1和SPC-A-1/cDDP等细胞株中该基因mRNA的表达。
- Objective To construct and evaluate the competitive quantitative RT PCR method for detecting the fusion gene of AML1 ETO in the patients of t(8;21)AML(acute myeloid leukemia). 目的 建立针对 AML- ETO融合基因转录本的竞争性定量反向 -聚合酶链反应 (RT- PCR)体系 ,并探讨其在 t(8;2 1)急性髓细胞性白血病 (acute myeloid leukemia,AML)微小残留病变跟踪中的应用价值。
- The levels of Prx and TrxR mRNA were determined 24 h and 48 h post,transfection by relative quantitative RT,PCR,and the growth of Trichomonas vaginalis was estimated under microscope 36 h post,transfection. 收集转染前、转染后24 h和48 h的毛滴虫细胞,用半定量反转录-PCR(RT-PCR)检测Prx和TrxR的mRNA水平;
- Fluorescent Quantitative RT - PCR 荧光定量RT-PCR
- real - time quantitative RT - PCR 实时定量RT-PCR
- real-time fluorescence quantitative RT - PCR 实时荧光定量RT-PCR
- Real - time fluorescence quantitative RT - PCR 实时荧光定量PCR
- Methods The expression of MDR1 gene in 77 cases of AL patients and 10 cases of control group was detected with semi quantitative reverse transcription polymerase chain reaction (RT PCR). 方法 采用半定量逆转录聚合酶链反应( R T? P C R),对77 例 A L病人及 10 例正常人 M D R1 基因表达进行检测。
- and AcrE was synthesized by 12 ploymerase chain reactions. Construction of internal standard of quantitative RT - PCR with synthesis method was quickly and easily accomplished. 合成法构建定量RT-PCR内标准DNA快捷、成功率高。
- RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
- Methods Plasma ETA was measured quantitively by RT PCR. 方法 应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
- Having no fixed quantitative value. 变量的没有固定数量值的
- Reverse transcriptase (RT)-polymerase chain reaction PCR) of Cdx-2. CDX-2的逆转录酶(RT)聚合酶链反应(PCR)技术。
- The semi drunk man regarded them with fishy eyes. 那个半醉汉迷迷糊糊地看着他们。
- Buying Signode Semi Automatic Strapping Machine. 伊朗求购半自动橡胶机械。
- Q: What do you call a Scouser in a three-bed semi? 你把一个在半独立式房屋中的利物浦人叫什么?
- Angiotensin receptor (ATR) mRNA expression of VSMC was measured by RT PCR. RT PCR检测VSMC血管紧张素受体 (ATR)mRNA表达。
- Our team has qualified for the semi ? final. 我们队已有资格进入准决赛。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- THIS SEMI CUSTOM HOME IS A PLEASER. 这个半自建独立屋是一个惊喜。
- 今日热词
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries