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- ScFv fusion protein 单链抗体融合蛋白
- The ScFv gene was expressed as fusion protein with phage g3p coat protein and displayed on the phage surface. 结果通过免疫荧光鉴定，这种抗体仍保留着亲代抗体的特性，能与登革3型病毒发生特异性结合。
- The L ScFv genes were inserted into the eukaryotic fusion protein expression vector pEGFP N3 and transfected into COS 7 cells to express respectively. 将所构建的单链抗体基因克隆入绿色荧光蛋白 (GFP)融合表达载体pEGFP N3,并转染COS 7细胞进行表达。
- Purified fusion protein was obtained. 得到纯化的融合蛋白。
- The GRA7 gene may be expressed as a GST fusion protein in E. coli. GRA7基因在大肠埃希菌中以GST融合蛋白的形式得到表达。
- To obtain the anti-transferrin receptor scFv and streptavidin(SA) recombinant fusion proteins which were high-efficiently and solubly expressed in E. 拟获得在大肠杆菌中能高效可溶表达的转铁蛋白受体单链抗体与链亲和素(SA)的重组融合蛋白。
- Human ScFv against botulinum neurotoxin serotype A(BoNTa) was modified by fusing human IgG1 Fc to C terminal of ScFv. ScFv-Fc fusion protein was expressed at high level over 30% of total host cell proteins in E. coli. 以获得的抗A型肉毒毒素单链抗体为模板,进行融合改构,将人IgG1的Fc片段连接到ScFv的C端,在大肠杆菌中实现抗体融合蛋白ScFv-Fc的表达,表达量30%25以上,蛋白以包含体形式存在,经过体外变复性的抗体融合蛋白ScFv-Fc,进行Prote in G Sepharose柱亲和层析纯化,纯度达90%25~95%25。
- The fusion protein was purified by GSTrap FF affinity chromatography. GSTrap FF亲和层析柱纯化融合蛋白;
- The fusion protein was purified through Nickel-affinity chromatography column. 用镍柱纯化融合蛋白。
- Methods The recombinant fusion protein MBP hEra was expressed in the E. 方法在大肠杆菌中,表达重组MBP?hEra融合蛋白。
- BVP22 can mediate intercellular trafficking of fusion protein in vitro and in vivo. 在体内外BVP22均能够介导与其融合的蛋白在细胞间发生穿梭。
- The titer of antiserum was detected by double agar immunodiffusion with GST-PI fusion protein. 并用双向免疫扩散法鉴定家兔多价抗血清的效价。
- EGF-Ang fusion protein had definitely cytotoxicity to Hep2 cells which over expressed EGFR in vitro. 融合蛋白EGF-Ang在体外对过度表达EGFR的Hep2细胞具有明显的杀伤作用。
- Recombinant TrxA/PEB1 fusion protein could be a valuable candidate for CJ vaccine with great applied value. （4）PEB1融合蛋白作为CJ的亚单位侯选基因工程疫苗不仅可行，而且有良好应用前景。
- Results: The CAT gene was cloned correctly and it's fusion protein was expressed in E.coli. 结果:成功克隆了CAT的基因片段,并在大肠杆菌中得到其融合蛋白的表达。
- A novel fusion protein namely IL- 2-Ang consisting of interleukin-2 ( IL-2 ) and angiogenin ( Ang) was expressed in E. 利用基因工程技术在大肠杆菌表达成功人白细胞介素2（IL－2）和人血管生成素（Angiogenin）的融合蛋白（IL－2－Ang）。
- The s-lap/GFP fusion protein can express in Hela cells and locate in entire cells, but express highly in the nucleus. s- lap/ GFP融合蛋白可在人 Hela细胞中表达 ,并主要定位于细胞核。
- Western blot proved that the recombinant protein is the fusion protein of APE/Ref|1. Western印迹证明重组蛋白为APE ref 1融合蛋白 .
- These fusion proteins were used to immunize rabbits to raise antisera. 这些表达产物用于免疫动物产生多克隆抗血清。
- Western-blot analysis with sera against CAV showed the fusion protein had good specificity. 在Western-blot免疫印迹分析中，3个融合蛋白与CAV阳性血清均能发生特异性反应，表明它们都具有良好的反应性。
