SDS-PAGE analysis indicated that Bombyx mori and Bombyx mandarina both showed a specific approximately 62 kD band compared with the control groups of empty BL21 and BL21 transformed by empty pET-28a.

• 然后通过Western blot分析表明在预期的约62 kD大小位置出现较明显的条带,而对照BL21菌和空pET-28a转化BL21菌都没有检测到条带,进一步证明了家蚕和野桑蚕CYP6B29得到了成功表达。 但在此预期的约62 kD大小位置以下出现了几条小分子量条带,而且家蚕和野蚕几乎完全相同。