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- Flow-through Rapid Hybridization and Reverse Dot Hybridization for Typing of HPV and Their Clinical Application 人乳头状瘤病毒快速导流杂交与传统杂交法比较基因分型及临床应用
- Reverse dot hybridization 反向点杂交
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- Effect of interferon on HBV determined by dot hybridization and PCR. HBeAg、HBV DNA斑点杂交以及定量PCR检测在评价干扰素抗HBV疗效的价值探讨
- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶链反应(PCR)结合反向斑点杂交(RDB)技术。
- EGFR gone transcription in rat embryo and adult tissues were analysed by RNA Northern and Dot hybridization using 2.4 Kb human EGFR eDNA pE 7 as probe. 本文报告用2.;4Kb EGFR cDNA PE7为探针;分析大鼠胚胎发育过程中全胚组织、成年大鼠七种组织和再生肝组织中EGFR基因转录产物的水平。
- Objective To evaluate specimen processing with sodium dodecyl(lauryl) sulfate(SDS) on the detection of Mycobacterium tuberculosis by PCR-reversed dot hybridization. 目的探讨应用十二烷基硫酸钠（SDS）处理标本在结核分支杆菌聚合酶链反应（PCR）中的价值。
- Using biotinylated human Y-specificDNA probe PHY2.1 and Cos 84, we carriedout Southern and dot hybridization withhuman DNA from artificially aborted cho-rionic villi. and with DNA of peripherallymphocytes from normal male and female. 作者以流产绒毛和外周血淋巴细胞DNA为材料;通过Southern印迹杂交和点杂交;考察了用生物素标记Y特异DNA探针PHY2.;1和Cos84进行产前性别诊断的可行性。
- We skillfully combinated PCR and reverse dot blot, using thehigh specificity of reverse dot blot overconming the deficiency ofhigh false positive rate of PCR as well as making use of thecharacteristic of high specificity of PCR. PCR-RDB检测方法将PCR技术和反向点杂交方法有机的结合起来，既利用了PCR技术灵敏度高的优点，又利用反向点杂交技术的高、度特异性克服了PCR方法假阳性率高的缺点，在实验室和现场的初步应用中都取得了较好的结果。
- Though RNA dot hybridization is negative, OP J18-1400 has a conservative domain of seven base pairs DNA sequence: 5-TTCCCTC-3, which is a homologous recombination hotspot domain in atp6 gene. 虽然OPJ18－1400对应的RNA斑点杂交呈阴性，但其DNA序列中存在一个七碱 基5－TTCCCTC－3的保守系列，它是atp6基因中同源重组热点区，其促使线粒体基因重组形成嵌
- However, by RNA dot hybridization the amount of mRNA produced in the transcription of parg-leuS was about 5 times that of the wild type le uS, and was similar to that of pKK-leuS, suggesting that the promoter of argS is very strong. 但是 ,RNA斑点杂交却发现从 parg leuS转录出来的leuSmRNA是野生型leuS转录的 5倍 ,几乎与从 pKK leuS中转录出来的量差不多 ,说明argS的启动子很强 ,接近于trp lac。
- The two recombinant plasmids were transfected into the HepG2 cells , then the inhibition effect on HBV replication by the genome of the HBV defective virus was studyed by ELISA dot hybridization and semiquantitative PCR. 构建含乙肝缺陷病毒基因组的重组逆转录病毒载体和含乙肝病毒基因组的重组逆转录病毒载体，共转染HepG2细胞，ELISA、PCR方法和斑点杂交初步研究乙肝缺陷病毒基因组对HBV复制的影响。
- Keywords Beta-thalassemia;minority;PCR;reverse dot blot（RDB） hybridization; 地中海贫血;少数民族;PCR-反向点杂交;
- PCR-flow through reverse dot blot PCR-流过式反向斑点杂交
- SCREENING MUTATIONS IN PHENYLKETONURIA BY MEANS OF NONRADIOACTIVE REVERSE DOT BLOT HYBRIDIZATION 非同位素逆相点杂交方法检测苯丙酮尿症突变基因
- Rapid detection of mycobacterium tuberculosis isoniazid-resistant genes by membrane reverse dot blot hybridization 应用膜反向斑点杂交技术检测耐异烟肼结核分支杆菌基因型
- Rapid identification of mycobacterium species by amplification of rpoB gene and reverse dot blot hybridization assay 应用rpoB基因PCR反向斑点杂交快速鉴定分枝杆菌菌种的研究
- Keywords thalassemia intermedia;polymerase chain reaction;reverse dot blot hybridization;prenatal diagnosis; 中间型地中海贫血;聚合酶链反应;反向斑点杂交;产前诊断;
- Our army suffered a slight reverse. 我们的部队受到轻微的挫败。
- The train arrived on the dot of three o'clock. 那辆火车三点钟准时到达。
