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- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- SCREENING MUTATIONS IN PHENYLKETONURIA BY MEANS OF NONRADIOACTIVE REVERSE DOT BLOT HYBRIDIZATION 非同位素逆相点杂交方法检测苯丙酮尿症突变基因
- Rapid detection of mycobacterium tuberculosis isoniazid-resistant genes by membrane reverse dot blot hybridization 应用膜反向斑点杂交技术检测耐异烟肼结核分支杆菌基因型
- Rapid identification of mycobacterium species by amplification of rpoB gene and reverse dot blot hybridization assay 应用rpoB基因PCR反向斑点杂交快速鉴定分枝杆菌菌种的研究
- Keywords thalassemia intermedia;polymerase chain reaction;reverse dot blot hybridization;prenatal diagnosis; 中间型地中海贫血;聚合酶链反应;反向斑点杂交;产前诊断;
- Rapid Typing of HPV by Reverse Dot Blot Hybridization and Its Clinical Characteristics Among Patients with Condyloma Acuminata 尖锐湿疣HPV导流杂交快速基因分型及临床意义
- Detection of rpoB mutations in rifampin resistant Mycobacterium tuberculosis strains by the reverse dot blot hybridization method 应用反向斑点杂交法检测耐利福平结核分支杆菌rpoB基因突变
- Reverse dot blot hybridization 反向点杂交
- Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶链反应(PCR)结合反向斑点杂交(RDB)技术。
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的:探讨斑点杂交法检测结核分枝杆菌的临床应用价值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑点杂交证实我们制备的探针是敏感而可靠的。
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法:采用聚合酶链反应结合等位基因特异的寡核苷酸探针杂交(PCR/ASO)技术。
- Methods:Using digoxigenin-labeled probe,75 sera of known or unknown etiology, and 17 blood donors were detected by dot blot hybridization. 方法:用地高辛素标记基因探针,斑点杂交法检测未知和已知病原的肝炎患者75例,正常对照17例。
- We skillfully combinated PCR and reverse dot blot, using thehigh specificity of reverse dot blot overconming the deficiency ofhigh false positive rate of PCR as well as making use of thecharacteristic of high specificity of PCR. PCR-RDB检测方法将PCR技术和反向点杂交方法有机的结合起来,既利用了PCR技术灵敏度高的优点,又利用反向点杂交技术的高、度特异性克服了PCR方法假阳性率高的缺点,在实验室和现场的初步应用中都取得了较好的结果。
- Methods b-FGF expressions were examined by RNA dot blot hybridization and immunohistochemical staining in specimens of 40 normal prostate tissues(NP)and 38 BPH tissues. 方法采用斑点杂交技术和免疫组化染色测定40例正常前列腺(NP)、38例前列腺增生症(BPH)组织中b-FGF的表达情况。
- Keywords Beta-thalassemia;minority;PCR;reverse dot blot(RDB) hybridization; 地中海贫血;少数民族;PCR-反向点杂交;
- The purpose of this study was evaluation of RT PCR technique,dot blot hybridization with PCR generated probe and SDS PAGE for the detection of rice black streaked dwarf ?Fijivirus?(RBSDV). 用RT PCR技术、PCR标记的探针点杂交和SDS PAGE检测了生产上严重危害玉米和水稻的水稻黑条矮缩病毒(RBSDV)。
- PCR-flow through reverse dot blot PCR-流过式反向斑点杂交