Results:1 .Establishing a method to separate gene fragments, combining with the RD technique with DNA silver staining, the results showed that in the PAGE gel, every group contained 7-10 clear DNA bands on average.
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- 将DNA银染技术与RD技术结合起来,分离基因片段,在PAGE胶上可发现,平均每组有7-10条DNA带,在日光灯下,带型清楚,可以快速分离EST片段,扩增后无杂带产生,直接可以用于载体克隆。