Results of DNA sequence analysis showed that nucleotide sequence of VK and VH of ScFv gene was similar to that of VK and VH of Fab gene, suggesting that there was no mutation in the construction of ScFv.

• DNA序列分析结果表明，pSCFV的 V K和 VH基因序列同 RRKZ的 V K和 VH基因序列完全相同，表明在Fab片段改建成ScFv过程中未发生基因突变。