Result: The polyploid of P.chinense was obtained using calli with stem treated with 0.2% colchicines medium,the inducing rate reached 12.45%.

• 结果:将茎段愈伤组织接种在含有0.；2%25秋水仙素的培养基上；通过培养形成的多倍体植株诱导率为12