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- Results 1. The result of restriction enzyme digest and gene sequence showed that the EWS-FLI-1 binding sequence (ACCGGAAGT) lay at the upper stream of diphtheria toxin A fragment. 结果 1、对重组载体的酶切鉴定和测序结果证实，在pS2-DTA中EWS-FLI-1特异性结合序列(ACCGGAAGT)位于白喉毒素A链基因上游。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
- Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. 利用酶切、AT克隆方法快速分析筛选出保护性抗原基因片段的重组子,从而制备成芯片探针。
- The ORF of two circadian gene BMAL1 and CRY1 were cloned from normal human whole brain marathon cDNA and verified with restriction enzyme digestion and DNA sequencing. 从正常人全脑marathon cDNA中克隆到人生物节律基因BMAL1和CRY1的开放读码框架(ORF)，经酶切和测序鉴定与GeneBank数据库中收录的序列基本一致。
- Methods Polymerase chain reaction with restrict enzyme digestion was used to detect the target gene polymorphism in 78 normotension controls and 72 EH patients. 目前认为，高血压是多种遗传因素和环境因素共同作用而引起的复杂性状疾病，在人类遗传易感性的基础上，在环境危险因素的作用下发病。
- Two fragments of genomic and mitochodrial DNAs were amplified by polymerase chain reaction (PCR). The mtDNA A1555G mutation was identified by the Prev-DAF Testing Kit and the GJB2 235delC mutation was detected by Apa I restriction enzyme digestion method. 血样经DNA提取,进行GJB2 235delC突变、线粒体DNA 12SrRNA A1555G突变的检测。
- PCR combined with restriction enzyme digestion PCR-双酶切分析
- Restriction enzyme digesting 限制性内切酶酶切
- The Restriction Enzyme Database from NEB. 限制苺资料库取自新英格兰生物实验室公司。
- Analysis of three lamivudine-resistant HBV mutants with the method of restriction enzyme digestion and its application 三种乙型肝炎病毒拉米夫定耐药突变的酶切分析方法及其应用
- Methods:The result of electrophoresis,purity and enzyme digesting of genome DNA of three kind of Pinaceae were compared. 方法:分别比较了用传统的CTAB法、SDS法以及3种改良的CTAB法提取的上述3种松科植物叶片基因组DNA的电泳、纯度和酶切效果。
- It enables a specific gene to be located on a particular restriction enzyme fragment. 它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。
- Methods By tryosin and DNA enzyme digest methods, we observed the human beings chorial trophcytes with light microscope and electronic microscope. 方法应用胰蛋白酶／DNA酶消化法进行消化，经光镜和电镜观察。
- Restriction Enzyme Digestion 限制片长多态性
- Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation. 根据揭示多态性的限制性内切酶的数量可将产生的突变大多归为点突变。
- Certain DNA sequences oriented within this gap are substrates for Alu I, a blunt end restriction enzyme. 如果用一段酶切断作为桥梁的DNA，那么整个电路的电流也被中断了。
- Amplified of the green fluent protein (GFP) gene from plasmid PCR3.1, and add suitable restriction enzyme cutting site. 从含有gfp基因的质粒pCR3.;1上扩增出全长基因，并且加上合适的酶切位点。
- Soya peptone is an enzyme digest of soya,this product contains carbohydrate,widely used in culture media and often used for the cultvation of many fastidious organisms and where rapid,luxuriant growth is required. 性状：本品系由大豆为原料，经酶消化而成，富含碳水化合物，广泛应用于微生物培养基的配制并常用于营养要求高的微生物培养及满足微生物快速大量生长的需要。
- The expressive vector pcDNA3.1(-)-ASL has been constructed and had been confirmed by restriction enzyme cut and sequencing. 构建的真核表达载体pcDNA3 .;1(-) ASL经过酶切鉴定和测序证实正确。
