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- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
- Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. 利用酶切、AT克隆方法快速分析筛选出保护性抗原基因片段的重组子,从而制备成芯片探针。
- Results 1. The result of restriction enzyme digest and gene sequence showed that the EWS-FLI-1 binding sequence (ACCGGAAGT) lay at the upper stream of diphtheria toxin A fragment. 结果 1、对重组载体的酶切鉴定和测序结果证实，在pS2-DTA中EWS-FLI-1特异性结合序列(ACCGGAAGT)位于白喉毒素A链基因上游。
- The ORF of two circadian gene BMAL1 and CRY1 were cloned from normal human whole brain marathon cDNA and verified with restriction enzyme digestion and DNA sequencing. 从正常人全脑marathon cDNA中克隆到人生物节律基因BMAL1和CRY1的开放读码框架(ORF)，经酶切和测序鉴定与GeneBank数据库中收录的序列基本一致。
- Methods Polymerase chain reaction with restrict enzyme digestion was used to detect the target gene polymorphism in 78 normotension controls and 72 EH patients. 目前认为，高血压是多种遗传因素和环境因素共同作用而引起的复杂性状疾病，在人类遗传易感性的基础上，在环境危险因素的作用下发病。
- Two fragments of genomic and mitochodrial DNAs were amplified by polymerase chain reaction (PCR). The mtDNA A1555G mutation was identified by the Prev-DAF Testing Kit and the GJB2 235delC mutation was detected by Apa I restriction enzyme digestion method. 血样经DNA提取,进行GJB2 235delC突变、线粒体DNA 12SrRNA A1555G突变的检测。
- PCR combined with restriction enzyme digestion PCR-双酶切分析
- Analysis of three lamivudine-resistant HBV mutants with the method of restriction enzyme digestion and its application 三种乙型肝炎病毒拉米夫定耐药突变的酶切分析方法及其应用
- The Restriction Enzyme Database from NEB. 限制苺资料库取自新英格兰生物实验室公司。
- Restriction Enzyme Digestion 限制片长多态性
- Restriction enzyme digesting 限制性内切酶酶切
- It enables a specific gene to be located on a particular restriction enzyme fragment. 它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。
- The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes. 酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
- Epithelial depuration was carried out by integration of burning and enzyme digestion. 细胞形态观察和电镜检测鉴定细胞。
- Both enzyme digestion and alkali treatment are effective methods of cell rupture, but each has a major disadvantage. 酶消化法和碱处理法都是细胞破碎的有效方法，但是也都有各自的缺点。
- Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation. 根据揭示多态性的限制性内切酶的数量可将产生的突变大多归为点突变。
- The cells were cultured by explant culture and enzymatic digestion. 组织化学染色和异种移植。
- Certain DNA sequences oriented within this gap are substrates for Alu I, a blunt end restriction enzyme. 如果用一段酶切断作为桥梁的DNA，那么整个电路的电流也被中断了。
- Amplified of the green fluent protein (GFP) gene from plasmid PCR3.1, and add suitable restriction enzyme cutting site. 从含有gfp基因的质粒pCR3.;1上扩增出全长基因，并且加上合适的酶切位点。
- The obtained bands were cloned into pGEM T-Easy vector, and the positive clone were confirmed by EcoR I enzyme digestion. 将所获片段克隆入pGEN T-Easy载体，EcoR I酶切鉴定。
