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- Methods Based on the suicide vector pGP704,a new screening in vivo-indu ced genes vector pGPcat was constructed with chloramphenicol a cetyltransferase(cat)gene as a report gene. 方法以自杀载体pGP704为骨架,用氯霉素乙酰转移酶基因作为报告基因,构建了用于筛选体内诱导基因的载体pGP-cat。
- Construction and expression of red fluorescent protein reporter gene vector containing human eNOS promoter 人内皮细胞一氧化氮合酶红色荧光蛋白报告基因载体的构建与表达
- Construction of reporter gene vector containing two promoters and its expressing activity assay in eukaryotic cells 双启动子报告基因表达载体的构建及其在真核细胞中表达活性测定
- Reporter gene vector 报告基因载体
- Constructing and Identifying the Firefly Luciferase Report Gene Vectors For 5'Flanking Regulated Elements of NGAL Gene NGAL基因5'侧翼区转录调控元件萤火虫荧光素酶报告基因表达载体的构建与鉴定
- Then it was inserted into pBI-L vector to construct HGF and Smad7 expression vector containing luciferase reporter gene and respond element of tetracycline (TRE) by recombinant DNA technology. 利用DNA基因重组技术，将目的片段DNA插入含有荧光素酶报告基因和四环素反应元件的真核表达载体pBI-L中，分别构建真核表达载体pBI-L-HGF和pBI-L-Smad7，并进行酶切和测序鉴定。
- Methods Green fluo rescence protein(GFP)gene was used as a reporter gene,and was transferred int o lens epithelial cells mediated by retroviral vector,GFP expression in cells w as analyzed. 方法用绿色荧光蛋白基因作为报告基因,以逆转录病毒载体携之转染晶状体上皮细胞,观察GFP在晶状体上皮细胞的表达情况。
- Objective To construct spinocerebellar ataxia 3 (SCA3) eukarotic expression vector with enhanced green fluorescent protein (EGFP) as report gene and to observe its expression in PC12 cells. 目的构建以增强型绿色荧光蛋白(EGFP)作为报告基因、带有脊髓小脑性共济失调3型(SCA3)基因的真核细胞表达载体,并转染PC12细胞,观察外源基因的表达情况。
- Objective To study hGH gene expression in the hypophysectomized rats using PVP as the gene vector. 目的以人工合成多聚物5%25聚乙烯吡咯烷酮(PVP)为基因治疗载体,研究人生长激素基因骨骼肌肌肉注射方法在生长激素(GH)缺乏症模型鼠中的表达。
- Thus we constructed reporter vectors in which the reporter gene is luciferase. 经酶切鉴定以及测序分析确定载体构建正确。
- Fig.1 The Construction of plant transformation vector of anti-sense gene of RbThe transcript director of Rb is opposite to 35S promoter.Thisvector has GUS report gene and hygromycin resistance gene. 标题： 图1 Rb反义基因表达载体结构示意图此表达载体Rb的转录方向与35S启动子的转录方向相反,并且含有GUS报道基因和抗潮霉素基因。
- Firefly luciferase reporter gene (fluc) vector 荧光素酶报告基因载体
- SuperLight Luciferase Reporter Gene Assays for gene regulation and modulator screen. 荧光素酶报告基因检测试剂盒,用于基因调控筛查的。
- This context concludes many researches about ultrasound microbubble: a novel gene vector in recent years.These researches include functional mechanism, influence factor, transfect... 本文对近年来有关超声微泡造影剂作为基因转染载体的作用机制、影响因素、转染实验和应用等方面的研究作了总结。
- This promoter is shown to direct the specific expression of the reporter gene,B-glucuronidase(GUS), in trichomes of Arabidopsis. 将此启动子与gus报告基因相连构建植物表达载体并转基因拟南芥，GUS组织化学定位分析表明此启动子在拟南芥叶片和茎的表皮毛细胞中特异表达。
- The transient transfection of Stat 3 cDNA into COS7 cells increased Stat3 protein level and the expression of the reporter gene. Stat3cDNA和报道基因在COS7细胞中的共转染实验表明 ,外源的Stat3蛋白具有DNA结合活性 ,并可增强报道基因的表达。
- Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation. 将启动子片段分别以不同方向与gus报告基因和nos终止子融合 ,构建了瞬时表达载体。
- Transient transfection of Stat3 cDNA into COS7 cells increased Stat3 protein level and also the reporter gene expression. Stat3 cDNA和报告基因在COS7细胞中的共转染实验表明，外源的Stat3蛋白具有DNA结合活性，并可增强报告基因的表达。
- The reporter had a red-hot story. 那个记者掌握著最新消息。
- Reporter gene imaging is an important imaging technique in molecular imaging,which can image various biological and genetical processes. 报告基因显像技术是分子影像学中重要的显像技术,其可对多种不同的生物学和分子遗传学过程进行显像,有望更快地应用于临床。
