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- Biosafety and expression of reporter gene gus in transgenic plants 转基因植物中报告基因gus的表达及其安全性评价
- Reporter gene GUS 报告基因GUS
- This promoter is shown to direct the specific expression of the reporter gene,B-glucuronidase(GUS), in trichomes of Arabidopsis. 将此启动子与gus报告基因相连构建植物表达载体并转基因拟南芥,GUS组织化学定位分析表明此启动子在拟南芥叶片和茎的表皮毛细胞中特异表达。
- Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation. 将启动子片段分别以不同方向与gus报告基因和nos终止子融合 ,构建了瞬时表达载体。
- By fusing this two fragment with gus reporter gene and nopaline terminator, we get two transient expression vectors which named pCAMBIA-ACTP and pBI-ACTPS. 将该启动子两个片段分别与gus报告基因和nos终止子融合,构建成瞬时植物表达载体pCAMBIA-ACTP和pBI-ACTPS。
- Seed chip GUS assay and herbicide leaf painting and spraying assays were applied to test the gus reporter gene and the herbicide resistant bar selectable marker gene, respectively. 分别采用种子切片GUS染色方法和除草剂涂抹以及喷洒方法检测gus报告基因和抗除草剂bar基因在后代的表达。
- Histochemical staining of GUS activity and in situ hybridization of pea lectin showed that gus reporter gene and pea lectin gene were expressed at translational level in transgenic tobacco hairy roots. GUS组织化学染色、蛋白免疫原位杂交证明 ;gus报告基因和豌豆凝集素基因已在转基因烟草发根中得到了转译表达 .
- PCR analysis and histochemical assay of GUS activity are conducted to testify the transformation events and the successful expression of reporter gene in independent hygromycin resistant calli. 为建立银杏遗传转化的高效体系,对一些重要的影响银杏转化的因素,包括外植体类型,选择标记和筛选压,农杆菌工程菌株,共培养时间和恢复时间第一次进行了试验并优化了条件。
- Fig.1 The Construction of plant transformation vector of anti-sense gene of RbThe transcript director of Rb is opposite to 35S promoter.Thisvector has GUS report gene and hygromycin resistance gene. 标题: 图1 Rb反义基因表达载体结构示意图此表达载体Rb的转录方向与35S启动子的转录方向相反,并且含有GUS报道基因和抗潮霉素基因。
- Thus we constructed reporter vectors in which the reporter gene is luciferase. 经酶切鉴定以及测序分析确定载体构建正确。
- SuperLight Luciferase Reporter Gene Assays for gene regulation and modulator screen. 荧光素酶报告基因检测试剂盒,用于基因调控筛查的。
- Promoter Trapping by Using Promoterless GUS Reporter Gene in Rice 用无启动子的GUS报告基因捕获水稻基因启动子
- The transient transfection of Stat 3 cDNA into COS7 cells increased Stat3 protein level and the expression of the reporter gene. Stat3cDNA和报道基因在COS7细胞中的共转染实验表明 ,外源的Stat3蛋白具有DNA结合活性 ,并可增强报道基因的表达。
- Transient transfection of Stat3 cDNA into COS7 cells increased Stat3 protein level and also the reporter gene expression. Stat3 cDNA和报告基因在COS7细胞中的共转染实验表明,外源的Stat3蛋白具有DNA结合活性,并可增强报告基因的表达。
- Reporter gene imaging is an important imaging technique in molecular imaging,which can image various biological and genetical processes. 报告基因显像技术是分子影像学中重要的显像技术,其可对多种不同的生物学和分子遗传学过程进行显像,有望更快地应用于临床。
- The minor T allele significantly attenuates reporter gene expression (P<0.01) and is impaired in its capacity to form DNA-protein complexes (P<0.05). 该等位基因处如为T可使基因表达量显著下降,并损伤其形成DNA-蛋白复合体的能力。
- The reporter had a red-hot story. 那个记者掌握著最新消息。
- SarA exhibiting an up-regulation on lipA and zinC, and down-regulation on atlE were demonstrated by reverse-transcription PCR and reporter gene lacZ analysis respectively. RT-PCR和lacZ报告基因的分析结果显示;在表皮葡萄球菌ATCC35984中;SarA对atlE(自溶酶基因)表达起负调控作用;而对lipA(脂肪酶基因)和zinC(膜相关锌金属蛋白酶基因)的表达则有正调控作用.
- GFP reporter gene under the direction of HBV promoter(enhancer) could be expressed in tumor cells and be expressed definitely and specifically in HCC Bel7402 cell. HBV启动子调控下的GFP报告基因能够在肝癌Bel740 2细胞中表达并具有一定的专一性。
- Plasmids pRecAgfp and pUvrAgfp were constructed after DNA damage-inducible promoters of recA and uvrA from Escherichia coli were fused to the reporter gene gfpmut3a operon. 将报告基因绿色荧光蛋白基因(gfpmut3a)分别置于recA和uvrA启动子调控下;构建成质粒pRecAgfp和pUvrAgfp;并转化E.