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- 脉络宁组40例(男31例,女9例;年龄62±s8a)用脉络宁20mL+10%葡萄糖500mL,静滴,qd,15d为一个疗程,用2个疗程程。Mailuoning(MLN) group including 40 patients (M 31. F 9; age 62+ Sa) was given MLN 20 mL in 10%25 glucose 500 mL, iv, qd, 15 d as a course of treatment;
- 最佳发酵条件为:培养温度:25℃,起始pH:5.0,500ml三角瓶装液量为200ml,摇床转速:150r/min,发酵培养12天。The most moderate breeding condition for fermentation is: Temperature 25C,the original pH for medium is 5. 0, The volume of fermentation solution is 200ml in 500ml Erlenmeyer flash , the rocking bed velocity is 150r/min, fermentation time is 12 days.
- 后一种方法是把195g PbO加到500ml 2mol/L的高氯酸溶液中,再通入电流密度为2.5mA/cm2的电流,取出沉积物,将其捣碎并用水洗净。In the latter method lead monoxide (195g) was added to 500 ml of 2M perchloric acid solution and a current of density 2.5mA cm-2 was passed. The deposit was removed, ground, and washed with water.
- 对表达人骨形成蛋白?2A(BMP?2A)的重组大肠杆菌YK537/pDH?B2m在500ml摇瓶中进行了培养条件的摸索实验,继后用5L自控发酵罐进行分批培养和分批补料培养,以获取rhBMP?2A。The optimization of cultivation condition in 500ml shake flasks was carried out to produce recombinant human bone morphogenetic protein 2A(BMP 2A)in recombinant Escherichia coli YK537/pDH B2m,followed by 5L fermentor batch and condition controlled fed batch culture to obtain BMP 2A.
- 结果表明,其最佳浸提方案是:成品茶样经粉碎过40目(0.45mm)筛,称取样品3g茶样用400ml沸蒸馏水冲泡,置于100℃恒温水浴锅中浸提30min后抽滤定容至500ml,而后用茚三酮比色法测定其含量。Results showed that the best extracted design was: the baked green tea was sifted through a 0.45 mm sieve, the 3 g of sample tea were infused by 400 ml boiling deionized water, and extracted in boiling water bath for 30 min and then the extraction was sucked with suction pump and settled to the volume of 500 ml. The FAA contents were determined by the ninhydrin colorimetric method.