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- Recombinant virus vector 重组病毒载体
- Objective To investigate the effect of recombinant aden o-associated virus vector(AVV) expressing the human tissue kallikrein(HK)ge ne (rAVV/HK) on human umbilical vein endothelial cells(HUVECs) in vitro. 目的 探讨重组人组织型激肽释放酶 (HK)基因腺相关病毒载体 (rAVV/HK)对体外培养的人脐静脉内皮细胞 (HUVECs)作用。
- The recombinant shuttle vector pBacPAK-CTB and pBacPAK-4aa-CTB was co-infected with Bm-BacPAK6 DNA into BmN cells. The recombinant virus was screened, plaque-purified and identified by PCR and Southern hybridization. 重组转移载体DNA与经Bsu36I酶切线性化的修饰型病毒Bm-BacPAK6 DNA共转染家蚕BmN细胞，然后经空斑纯化、PCR扩增、Southern杂交等方法鉴定出含CTB基因的重组病毒rBm-BacCTB和rBm-Bac4aa-CTB。
- Results Recombinant virus with high titre, prepared by this method, could be... 结果用本方法制备出了较高效价的重组病毒,并能在哺乳细胞中很好地表达。
- According to Baker, the vaccine for smallpox virus vector HIV immunogen. 据贝克介绍,疫苗用天花病毒作艾滋病病毒免疫原载体。
- recombinant adeno-associated virus vector 重组腺伴随病毒载体
- The green fluorescence protein (GFP) gene recombinant virus labeling is a new method for the neuroanatomical studies. 绿色荧光蛋白(GFP)基因重组病毒标记技术是神经解剖研究的新方法。
- AIM: To investigate the protein changes in immature dendritic cells (DC) infected with sendai virus vector. 目的:研究仙台病毒载体对未成熟树突状细胞(DC)蛋白质表达的影响.
- The recombinant virus CAV2-TSOL18m was obtained and the classical cytopathic effects(CPE) of CAV-2 could be observed . Asc I+Cla I双酶切pCAV2-TSOL18m释放重组基因组，转染DK细胞，获得了重组病毒CAV2-TSOL18m。 该重组病毒在DK细胞上能产生典型的腺病毒细胞病变。
- Gene transfer vector is the important ingredient in gene therapy,which includes virus vector and non-virus vector. 基因转移载体是基因治疗的重要组成部分,包括病毒载体和非病毒载体。
- When the larve was infected with recombinant virus, the liquefaction couldn't be observed. 用所得到的重组病毒感染虫体,发现虫体不液化。
- Stable Integration of Genomic Gene of Interest in Mammalian Cells Following Adeno-associated Virus Vector Mediated Gene Transfer. 腺相关病毒介导基因组来源的基因转移与整合。
- The hBMP-7 recombinant adeno-associated virus vectors can be successfully constructed in vitro and rAAV2-hBMP7 can infect ADAS cells. 成功构建了hBMP7基因重组腺相关病毒载体和培养出大鼠ADAS cells,rAAV2-hBMP7载体在体外可高效转染大鼠ADAS cells。
- This paper reviews two basic types of epitope presentation and polypeptide expression systems, and discusses the development and application prospect of plant virus vector. 文章主要介绍了抗原展示系统和多肽表达系统，并分别列举一些植物病毒作为表达载体的研究进展情况以及应用前景。
- Bioassay results showed that the recombinant virus caused a 26% higher mortality at 60 hpi than that of its wild-type parental virus against the larvae of the beet armyworm, Spodoptera exigua. 生物测定表明，该重组病毒感染甜菜夜蛾的毒力超过野生型亲本毒株AcMNPV，特别是在感染幼虫后60小时之前，感染幼虫的平均死亡率高于亲本毒株26%25。
- And the international AIDS pandemic vaccine design and technology very different routes, China's ongoing clinical trials is replicating vaccinia virus vector, belonging to the vaccine. 与国际上流行的艾滋病疫苗设计理念和技术路线大不相同，中国正在进行临床试验的是复制型痘苗病毒载体，属于活疫苗。
- Plaque assays were done to isolate those white recombinant clones for rhBMP2 and 3. PCR analyses of viral DNAs were done to identify the target DNAs in recombinant viruses . 空斑形成后，经X-gal染色，挑取白色的单个重组克隆保存，取B2和B3各5个白色克隆进行第一轮扩增，收集病毒上清。
- Objective In order to obtain basic information of distribution of Dengue viruses vector mosquitoes:Aedes albopictus in north and northwest of Hebei province. 目的了解登革热主要传播媒介白纹伊蚊在河北省西部、北部的分布,为制定控制登革热等蚊媒疾病方案提供基础资料。
- Preparation of Stabilizer of Freeze-dried Recombinant MVA Virus Vector Vaccine 重组MVA病毒载体疫苗的冻干保护剂研究
- When we tested recombinant viruses in a tissue culture of human lung cells, we found that a virus with the 1918 NS1 gene was indeed more effective at blocking the host's type I IFN system. 当我们将重组的病毒在人类肺细胞的组织培养中测试时，发现带有1918年NS1基因的病毒，确实能更有效地阻断宿主的第一型干扰素系统。
