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- Keywords Mycobacterium tuberculosis ESAT 6 Recombinant shuttle plasmid PCR amplification; 结核分枝杆菌;ESAT-6;重组穿梭质粒PCR扩增;
- A novel shuttle plasmid which can be replicated in yeast and expressed in mammal cell is required in oral gene therapy with yeast as vector to deliver target gene. 为研究酵母作为载体在口服基因治疗及免疫中的作用,需要一种能够在酵母中复制而在哺乳动物细胞中表达的穿梭载体。
- The recombinant shuttle vector pBacPAK-CTB and pBacPAK-4aa-CTB was co-infected with Bm-BacPAK6 DNA into BmN cells. The recombinant virus was screened, plaque-purified and identified by PCR and Southern hybridization. 重组转移载体DNA与经Bsu36I酶切线性化的修饰型病毒Bm-BacPAK6 DNA共转染家蚕BmN细胞,然后经空斑纯化、PCR扩增、Southern杂交等方法鉴定出含CTB基因的重组病毒rBm-BacCTB和rBm-Bac4aa-CTB。
- Enzyme Picture of Secrete Shuttle Plasmid P~# GTE_6 DNA of E.coli 大肠杆菌分泌型穿梭质粒PGTE_5DNA的酶切图谱
- Keywords Yeast;Shuttle plasmid;Transformation efficiency; 酿酒酵母;穿梭质粒;转化率;
- Keywords Zymomonas mobilis;Shuttle plasmid;Stability;Copy number; 运动发酵单胞菌;穿梭质粒;稳定性;拷贝数;
- Shuttle plasmid construction of Signature-Tagged Mutagenesis technology 信号标签诱变突变体文库穿梭质粒构建
- Construction of regulatory genes in broad host range shuttle plasmid 构建同源调节基因的广谱穿梭质粒
- Construction and Transformation of Bacteria-Yeast Shuttle Plasmid pGBKT7-MIF 细菌-酵母穿梭表达质粒pGBKT7-MIF的构建及转化
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- The Construction of Shuttle Plasmid Vector of Streptomyces and E. colt 链霉菌和大肠杆菌穿梭质粒载体的构建
- Keywords Signature-tagged mutagenesis;Shuttle plasmid;Mutant library; 信号标签诱变;穿梭质粒;突变体文库;
- The U.S. made the first space shuttle in the world. 美国制造了世界上第一架航天飞机。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
- The space shuttle will orbit a weather satellite. 太空梭将把一个气象卫星送入运行轨道。
- I'm flying to Boston on the shuttle. 我将乘穿梭班机去波士顿。
- The buses shuttle visitors to Disneyland. 区间公共汽车将游客载至迪尼斯乐园。
- Construction of the shuttle plasmid vector for fusion protein gene and expression in Hepatocellular Carcinoma cells 携带融合基因穿梭质粒的构建及在人肝癌细胞中的表达
- Construction and identification of shuttle plasmid expressing ICL-GFP fusion protein in Mycobacterium 分枝杆菌融合表达ICL-GFP穿梭质粒的构建及鉴定
- MUTAGENESiS STUDY OF EMS TO SHUTTLE PLASMID PZ189 AND THE MUTANT SUPF GENE DETECTION BY TGGE TECHNIQUE 甲基磺酸乙酯对穿梭质粒P~(Z189)的诱变作用及温度梯度凝胶电泳对SUP~F突变基因的检测
- 今日热词
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries