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- A recombinant expressing plasmid pCMV4/TPO was also constructed by cloning the rhTPO cDNA in an eukaryotic expressing vector pCMV4. When the recombinant plasmid was used to transfect the COS7 cells, temporary expression of rhTPO was detected. 将TPOcDNA亚克隆至哺乳动物细胞瞬时表达载体pCMV4，形成了重组表达质粒pCMV4/TPO。 以该重组质粒转染COS7细胞，可测到TPO在COS7细胞中的瞬时表达
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
- Methods Murine IL 12 cDNA was inserted into optimized HBcAg DNA vaccine pST HBc. The resultant recombinant expression plasmid pST HBc/IL12 was transfected into COS7 cells and its expressed product was detected by ELISA. 方法 将小鼠IL 12基因插入结构优化的DNA疫苗pST HBc,构成pST HBc/IL12 ,将pST HBc/IL12转染COS7细胞 ,ELISA检测培养上清中的HBcAg和小鼠IL 12。
- The whole FC cDNA was cloned into pET-28a (+) containing T7 promoter and recombinant expression plasmid pET-FC was constructed. The recombinant plasmid was transformed into E. coli BL21(DE3). 将分段克隆得到的两段FC片段经相应酶切后 ,与含T7启动子的质粒 pET 2 8a(+)在一个体系中作连接反应 ,构建表达质粒pET2 8a FC ,转化大肠杆菌BL2 1(DE3) ,筛选表达菌株。
- The result of immunologic test showed that there was crossed protection between the two bacteria.Vanhnaseng(2004) cloned the gene of cIL-18 and obtained recombinant expression plasmid pcDNA3.1/cIL-18. 曹素芳（2004）克隆了禽巴氏杆菌外膜蛋白H基因（ompH），构建了该基因与cIL-18基因真核共表达质粒pcDNA3.;1/ompH-cIL-18，免疫试验结果表明，pcDNA3
- For the first time to recombine ALR procaryotic expression vector, and get the pET28a-hALR recombined expressing plasmid which was tested by sequence analysis. 首次构建了重组ALR原核表达载体，获得了pET28a-hALR的重组表达质粒，并测序证实。
- Plant recombinant expression plasmid 植物表达载体
- The results showed that eaeA gene was correctly inserted into the pET-28a(+) vector, and the recombinant expression plasmids (named pET-eaeA) were constructed successfully. 将经质粒PCR鉴定和双酶切鉴定为阳性的转化子测序作进一步鉴定，成功构建了重组表达质粒pET-eaeA。
- Recombinant expressing plasmid 重组表达质粒
- The sequences coding two target proteins were cloned into the above recombinant expression plasmids separately and transfected into Cos 7 cells mediated via liposome. The expression products were detected by Western blot and co immunoprecipitation. 将两种候选蛋白的编码序列分别克隆于上述融合载体,转染Cos?7细胞后的表达产物,可利用抗HA或myc的mAb进行Western?blot和免疫共沉淀。
- STⅡ plant recombination expression plasmid 植物重组表达质粒
- Construction of the Recombinant Expression Plasmid for the Expression of the Whole Anti-D Immunoglobulin Molecules 完整人抗-D抗体分子表达载体的构建
- Identification of recombinant expression plasmid by restriction endonuclease digestion 重组表达质粒酶切鉴定
- A STUDY OF GENE IMMUNIZATION WITH RECOMBINANT EXPRESSION PLASMID OF EPSTEIN-BARR VIRUS MEMBR ANEANTIGEN 含有Epstein-Barr病毒膜抗原的重组表达质粒及其基因免疫
- Keywords human herpesvirus 8;K12 gene;green fluoresent protein;recombinant expression plasmid; 关键词人疱疹病毒8型;K12基因;绿色荧光蛋白;重组表达质粒;
- In order to get recombinant BPI, the gene was cloned into an expressing plasmid and expressed in CHO cells. 构建真核表达质粒 ,在CHO细胞中实现BPI基因的稳定表达。
- Keywords Oenococcus oeni;malolactic enzyme gene;malate permease gene;recombinant expression plasmid;expression;Saccharomyces cerevisiea; 酒酒球菌;苹果酸-乳酸酶基因;苹果酸通透酶基因;重组表达质粒;表达;酿酒酵母;
- Objective To construct mouse granulocyte-macrophage colony stimulating factor (mGM-CSF) gene eukaryotic expressing plasmid pcDNA3-GM-CSF, to transfect the recombinant into erythroleukemia cell line FBL-3, and identify their biological activity. 目的 构建小鼠转粒细胞 巨噬细胞集落刺激因子 (mGM CSF)真核表达质粒 pcDNA3 GM CSF ,转染红白血病细胞系FBL 3,并鉴定其活性。
- The recombinant eukaryotic expression plasmid,pcDNA3.1A DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. 成功构建重组真核表达质粒 pcDNA3 .;1A DCN;转染MsC并筛选出 2个阳性克隆株。
- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
